Principle Of Fluorescence Activated Cell Sorting Technique

Fluorescence activated cell sorting facs is a specialized type of flow cytometry.

Principle of fluorescence activated cell sorting technique.

A description of fluorescence activated cell sorting of live cell populations. Facs is useful for applications such as establishing cell lines carrying a transgene enriching for cells in a specific cell cycle phase or studying the transcriptome or genome or proteome of a whole population on a single cell level. In a facs fluorescence by a cell induces the device to put a charge on a droplet of the transporting fluid containing that cell. The molecules can be receptors or protein glycoprotein or lipid surface antigens.

Fluorescence activated cell sorting facs is a powerful method to physically select a subset of target cells from a mixed population. It provides a method for sorting a heterogeneous mixture of biological cells into two or more containers one cell at a time based upon the specific light scattering and fluorescent characteristics of each cell. Fluorescence activated cell sorting facs of live cells separates a population of cells into sub populations based on fluorescent labeling. Fluorescence activated cell sorting of live cells.

By utilizing highly specific antibodies labeled with fluorescent conjugates facs analysis allows us to simultaneously collect data on and sort a biological sample by a nearly limitless number of different parameters. It provides a method for sorting a heterogeneous mixture of biological cells into two or more containers one cell at a time based upon the specific light scattering and fluorescent characteristics of each cell. Fluorescent activated cell sorting of environmental samples containing microalgae. Facs is an abbreviation for fluorescence activated cell sorting which is a flow cytometry technique that further adds a degree of functionality.

This strategy is used when the target cell expresses a specific cell surface molecule. The charge is specific to the wavelength of the fluorescent light which allows for differential sorting by those different charges. Cells are restricted to a narrow band by a liquid stream sheath liquid in the flow cell.